Endothelial nitric oxide synthase haplotypes affect the susceptibility to hypertension in patients with type 2 diabetes mellitus.

Atherosclerosis. 2006 ; 189(1):241-6

Sandrim VC, de Syllos RW, Lisboa HR, Tres GS, Tanus-Santos JE.

Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, University of
Sao Paulo, Av. Bandeirantes, 3900, 14049-900 Ribeirao Preto, SP, Brazil.

Type 2 diabetes mellitus (T2DM) and hypertension (HT) commonly coexist. While endothelial nitric oxide synthase (eNOS) haplotypes have been associated with HT, it is unknown whether eNOS genotypes/haplotypes are associated with altered susceptibility to HT in patients with T2DM. We studied the distribution of three eNOS genetic polymorphisms: a single nucleotide polymorphism in the promoter region (T(-786)C), in exon 7 (Glu298Asp), and a variable number of tandem repeats in intron 4(b/a). Genotypes were determined for 102 healthy controls, 119 patients with HT, 66 patients with T2DM, and 113 patients

A polymorphism in the delta-aminolevulinic acid dehydratase gene modifies plasma/whole blood lead ratio

Arch Toxicol. 2006; 80(7):394-8

Montenegro MF, Barbosa F Jr, Sandrim VC, Gerlach RF, Tanus-Santos JE.

Delta aminolevulinic acid dehydratase (ALAD) plays an important role in lead poisoning. This study was carried out to examine the effects of ALAD gene polymorphism (G177C) on %Pb-P(plasma lead)/Pb-B(whole blood) ratio in 142 subjects environmentally exposed to lead. Genotypes for the ALAD G177C polymorphism were determined by PCR and restriction fragment length digestion. Pb-P and Pb-B were determined by inductively coupled plasma mass spectrometry and by graphite furnace atomic absorption spectrometry, respectively. The allele frequencies for ALAD1 and ALAD2 alleles were 0.897 and 0.103, respectively. We combined both ALAD 1-2 and ALAD 2-2 genotypes together (ALAD 1-2/2-2 group) and

Cada vez mais perto do etanol celulósico

Empresa traz para o Brasil enzimas capazes de auxiliar na produção de álcool a partir do bagaço de cana

 

A produção do etanol celulósico - o etanol extraído a partir da biomassa, como o bagaço de cana, por exemplo - pode finalmente ganhar escala industrial. Esse processo, que já é estudado há pelo menos uma década no mundo, pode aumentar em pelo menos um terço a produção de etanol no País, atualmente em 24 bilhões de litros, e tornar mais próxima a consolidação do mercado externo de biocombustíveis.

Reportagem do ESTADÃO - leia mais em: http://www.estadao.com.br/noticias/suplementos,cada-vez-mais-perto-do-etanol-celulosico,531765,0.htm

eNOS genotype-dependent correlation between whole blood lead and plasma nitric oxide products concentrations.

Nitric Oxide. 2006; 14(1):58-64

Barbosa F Jr, Sandrim VC, Uzuelli JA, Gerlach RF, Tanus-Santos JE.

Experimental data indicate that lead exposure decreases nitric oxide (NO) availability. However, no previous study has examined whether lead exposure affects plasma nitrite/nitrate (NO(x)) concentrations in humans. In addition, the T(-786)C polymorphism affects endothelial NO synthase (eNOS) expression and endogenous NO release. Here, we investigated whether there is an association between the circulating concentrations of NO(x) and the concentrations of lead in whole blood (B-Pb) and in plasma (P-Pb) from lead-exposed

Cientista tailandês cria enzimas que reciclam papel laminado

Combinação de proteínas recupera polpa de material usado em caixa de cigarro e etiquetas.

Acesse o link e confira!!!!

http://noticias.r7.com/tecnologia-e-ciencia/noticias/cientista-tailandes-cria-enzimas-que-reciclam-papel-laminado-20101207.html

Susceptible and protective eNOS haplotypes in hypertensive black and white subjects

Atherosclerosis. 2006; 186(2):428-32

Sandrim VC, Coelho EB, Nobre F, Arado GM, Lanchote VL, Tanus-Santos JE.

Polymorphisms in the endothelial nitric oxide synthase (eNOS) gene have been inconsistently associated with hypertension. This inconsistency may derive from population stratification secondary to ethnic diversity, and consideration limited to only one rather than combinations of polymorphisms. We studied three genetic variations in the eNOS gene: a single nucleotide polymorphism in the promoter region (T-786C), in exon 7 (Glu298Asp), and a variable number of tandem repeats in intron 4 (b/a) of the eNOS gene in hypertensives (112 whites and 91 blacks) and normotensives (113 whites and 87 blacks). In addition, we also examined the

Consistent interethnic differences in the distribution of clinically relevant endothelial nitric oxide synthase genetic polymorphisms

Nitric Oxide. 2005; 12(3):177-82

Marroni AS, Metzger IF, Souza-Costa DC, Nagassaki S, Sandrim VC, Correa RX, Rios-Santos F, Tanus-Santos JE.

A maldistribution of endothelial nitric oxide synthase (eNOS) genetic variants may explain differences in NO-mediated effects and response to drugs among black and white subjects. While interethnic differences in the distribution of eNOS genetic variants exist in the American population, it is not known whether such interethnic differences exist in other populations. To test this possibility, we examined the distribution of genetic variants of three clinically relevant eNOS polymorphisms (T(-786)C in the promoter, the VNTR in intron 4, and the Glu298Asp variant in exon 7) in 136 black and 154 white subjects from a Brazilian

Influence of temperature on the properties of the xylanolytic enzymes of the thermotolerant fungus Aspergillus phoenicis.

J Ind Microbiol Biotechnol. 2004 Feb;31(2):88-93

Rizzatti AC, Sandrim VC, Jorge JA, Terenzi HF, Polizeli MLTM

This study reports on the effects of growth temperature on the secretion and some properties of the xylanase and beta-xylosidase activities produced by a thermotolerant Aspergillus phoenicis. Marked differences were observed when the organism was grown on xylan-supplemented medium at 25 degrees C or 42 degrees C. Production of xylanolytic enzymes reached maximum levels after 72 h of growth at 42 degrees C; and levels were three- to five-fold higher than at 25 degrees C. Secretion of xylanase and beta-xylosidase was also  strongly stimulated at the higher temperature. The optimal temperature was 85 degrees C for extracellular and 90 degrees C for intracellular beta-xylosidase activity, independent of the growth temperature. The optimum temperature for extracellular xylanase increased from 50 degrees C to 55 degrees C when the fungus was cultivated at 42 degrees C. At the higher temperature, the xylanolytic enzymes produced by A. phoenicis showed increased thermostability, with changes in the profiles of pH optima. The chromatographic profiles were distinct when samples obtained from cultures grown at different temperatures were eluted from DEAE-cellulose and Biogel P-60 columns.

Transcrição

TRANSCRIÇÃO: é o processo de formação do RNA mensageiro(RNAm)  utilizando-se como molde a molécula de DNA. A molécula de RMAm tem como função "informar" ao RNA transportador (RNAt) a ordem correta dos aminoácidos a serem sintetizados e ligados formando as proteínas necessárias ao metabolismo celular.


Assunto super interessante e muito bem ilustrado pelo vídeo a seguir. Click no link abaixo.

http://www.youtube.com/watch?v=nNjbYfhfgIo

Video com autoria de terceiros.

Production and properties of xylanases from Aspergillus terricola Marchal and Aspergillus ochraceus and their use in cellulose pulp bleaching

M. Michelin • S. C. Peixoto-Nogueira • J. H. A. Betini • T. M. da Silva • J. A. Jorge • H. F. Terenzi • M. L. T. M. Polizeli

Bioprocess Biosyst Eng (2010) 33:813–821

Aspergillus terricola and Aspergillus ochraceus, isolated from Brazilian soil, were cultivated in Vogel and Adams media supplemented with 20 different carbon sources, at 30ºC, under static conditions, for 120 and 144 h, respectively. High levels of cellulase-free xylanase were produced in birchwood or oat spelt xylan-media. Wheat bran was the most favorable agricultural residue for xylanase production. Maximum activity  was obtained at 60ºC and pH 6.5 for A. terricola, and 65ºC and pH 5.0 for A. ochraceus. A. terricola

Cadeia respiratória e síntese de ATP

Assuntinho difícil que pode ser divertido! Click no link abaixo:

http://www.youtube.com/watch?v=ywH_lgRZtZQ

Autoria de terceiros

Purification and characterization of a thermostable a-amylase produced by the fungus Paecilomyces variotii

Michele Michelin, Tony M. Silva, Vivian M. Benassi, Simone C. Peixoto-Nogueira, Luiz Alberto B. Moraes, Juliana M. Leão, João A. Jorge, Héctor F. Terenzi, Maria de Lourdes T. M. Polizeli

Carbohydrate Research 345 (2010) 2348–2353

An a-amylase produced by Paecilomyces variotii was purified by DEAE-cellulose ion exchange chromatography, followed by Sephadex G-100 gel filtration and electroelution. The a-amylase showed a molecular mass of 75 kDa (SDS–PAGE) and pI value of 4.5. Temperature and pH optima were 60ºC and 4.0, respectively. The enzyme was stable for 1 h at 55ºC, showing a t50 of 53 min at 60 ºC. Starch protected the enzyme against thermal inactivation. The a-amylase was more stable in alkaline pH. It was activated mainly

Hemiceluloses e o sistema xilanolítico

As hemiceluloses são classificadas de acordo com o açúcar presente em sua molécula. Assim, a xilana é um homopolímero linear que contém monômeros de b-D-xilopiranosil unidos por ligações glicosídicas b-1,4 que, na natureza, geralmente está associada a outros açúcares, formando glucuronoxilanas, glucuronoarabinoxilanas, glucomananas, arabinogalactanas e galactoglucomananas. Devido a sua heterogeneidade estrutural, a degradação da xilana requer a ação de várias enzimas, ou seja, de um sistema enzimático que se encontra presente em fungos e bactérias. As enzimas pertencentes ao sistema xilanolítico

Enzimas: A Base Molecular da Vida

Clik no link abaixo e veja o vídeo extremamente interessante! Mostra uma aula sobre enzimas discutindo os principais conceitos deste assunto: o que é uma enzima, quais são seus co-fatores, inibidores, etc.


http://www.youtube.com/watch?v=DJf1YXCvD3s

Autoria de terceiros

Xylanases from Aspergillus niger, Aspergillus niveus and Aspergillus ochraceus produced under solid-state fermentation and their application in cellulose pulp bleaching

Bioprocess Biosyst Eng (2009) 32:819–824

J. H. A. Betini, M. Michelin, S. C. Peixoto-Nogueira, J. A. Jorge, H. F. Terenzi, M. L. T. M. Polizeli

This study describes the production of xylanases from Aspergillus niveus, A. niger, and A. ochraceus under solid-state fermentation using agro-industrial residues as substrates. Enzyme production was improved using a mixture of wheat bran and yeast extract or peptone. When a mixture of corncob and wheat bran was used, xylanase production from A. niger and A. ochraceus increased by 18%. All cultures were incubated at 30ºC at 70–80% relative humidity for 96 h. For biobleaching assays, 10 or 35 U of xylanase/g dry cellulose pulp were incubated at pH 5.5 for 1 or 2 h, at 55ºC. The delignification efficiency was 20%, the brightness (percentage of ISO) increased two to three points and the viscosity was maintained confirming the absence of cellulolytic activity. These results indicated that the use of xylanases could help to reduce the amount of chlorine compounds used in cellulose pulp treatment.