A polymorphism in the delta-aminolevulinic acid dehydratase gene modifies plasma/whole blood lead ratio

Arch Toxicol. 2006; 80(7):394-8

Montenegro MF, Barbosa F Jr, Sandrim VC, Gerlach RF, Tanus-Santos JE.

Delta aminolevulinic acid dehydratase (ALAD) plays an important role in lead poisoning. This study was carried out to examine the effects of ALAD gene polymorphism (G177C) on %Pb-P(plasma lead)/Pb-B(whole blood) ratio in 142 subjects environmentally exposed to lead. Genotypes for the ALAD G177C polymorphism were determined by PCR and restriction fragment length digestion. Pb-P and Pb-B were determined by inductively coupled plasma mass spectrometry and by graphite furnace atomic absorption spectrometry, respectively. The allele frequencies for ALAD1 and ALAD2 alleles were 0.897 and 0.103, respectively. We combined both ALAD 1-2 and ALAD 2-2 genotypes together (ALAD 1-2/2-2 group) and

Cada vez mais perto do etanol celulósico

Empresa traz para o Brasil enzimas capazes de auxiliar na produção de álcool a partir do bagaço de cana

 

A produção do etanol celulósico - o etanol extraído a partir da biomassa, como o bagaço de cana, por exemplo - pode finalmente ganhar escala industrial. Esse processo, que já é estudado há pelo menos uma década no mundo, pode aumentar em pelo menos um terço a produção de etanol no País, atualmente em 24 bilhões de litros, e tornar mais próxima a consolidação do mercado externo de biocombustíveis.

Reportagem do ESTADÃO - leia mais em: http://www.estadao.com.br/noticias/suplementos,cada-vez-mais-perto-do-etanol-celulosico,531765,0.htm

eNOS genotype-dependent correlation between whole blood lead and plasma nitric oxide products concentrations.

Nitric Oxide. 2006; 14(1):58-64

Barbosa F Jr, Sandrim VC, Uzuelli JA, Gerlach RF, Tanus-Santos JE.

Experimental data indicate that lead exposure decreases nitric oxide (NO) availability. However, no previous study has examined whether lead exposure affects plasma nitrite/nitrate (NO(x)) concentrations in humans. In addition, the T(-786)C polymorphism affects endothelial NO synthase (eNOS) expression and endogenous NO release. Here, we investigated whether there is an association between the circulating concentrations of NO(x) and the concentrations of lead in whole blood (B-Pb) and in plasma (P-Pb) from lead-exposed

Cientista tailandês cria enzimas que reciclam papel laminado

Combinação de proteínas recupera polpa de material usado em caixa de cigarro e etiquetas.

Acesse o link e confira!!!!

http://noticias.r7.com/tecnologia-e-ciencia/noticias/cientista-tailandes-cria-enzimas-que-reciclam-papel-laminado-20101207.html

Susceptible and protective eNOS haplotypes in hypertensive black and white subjects

Atherosclerosis. 2006; 186(2):428-32

Sandrim VC, Coelho EB, Nobre F, Arado GM, Lanchote VL, Tanus-Santos JE.

Polymorphisms in the endothelial nitric oxide synthase (eNOS) gene have been inconsistently associated with hypertension. This inconsistency may derive from population stratification secondary to ethnic diversity, and consideration limited to only one rather than combinations of polymorphisms. We studied three genetic variations in the eNOS gene: a single nucleotide polymorphism in the promoter region (T-786C), in exon 7 (Glu298Asp), and a variable number of tandem repeats in intron 4 (b/a) of the eNOS gene in hypertensives (112 whites and 91 blacks) and normotensives (113 whites and 87 blacks). In addition, we also examined the

Consistent interethnic differences in the distribution of clinically relevant endothelial nitric oxide synthase genetic polymorphisms

Nitric Oxide. 2005; 12(3):177-82

Marroni AS, Metzger IF, Souza-Costa DC, Nagassaki S, Sandrim VC, Correa RX, Rios-Santos F, Tanus-Santos JE.

A maldistribution of endothelial nitric oxide synthase (eNOS) genetic variants may explain differences in NO-mediated effects and response to drugs among black and white subjects. While interethnic differences in the distribution of eNOS genetic variants exist in the American population, it is not known whether such interethnic differences exist in other populations. To test this possibility, we examined the distribution of genetic variants of three clinically relevant eNOS polymorphisms (T(-786)C in the promoter, the VNTR in intron 4, and the Glu298Asp variant in exon 7) in 136 black and 154 white subjects from a Brazilian

Influence of temperature on the properties of the xylanolytic enzymes of the thermotolerant fungus Aspergillus phoenicis.

J Ind Microbiol Biotechnol. 2004 Feb;31(2):88-93

Rizzatti AC, Sandrim VC, Jorge JA, Terenzi HF, Polizeli MLTM

This study reports on the effects of growth temperature on the secretion and some properties of the xylanase and beta-xylosidase activities produced by a thermotolerant Aspergillus phoenicis. Marked differences were observed when the organism was grown on xylan-supplemented medium at 25 degrees C or 42 degrees C. Production of xylanolytic enzymes reached maximum levels after 72 h of growth at 42 degrees C; and levels were three- to five-fold higher than at 25 degrees C. Secretion of xylanase and beta-xylosidase was also  strongly stimulated at the higher temperature. The optimal temperature was 85 degrees C for extracellular and 90 degrees C for intracellular beta-xylosidase activity, independent of the growth temperature. The optimum temperature for extracellular xylanase increased from 50 degrees C to 55 degrees C when the fungus was cultivated at 42 degrees C. At the higher temperature, the xylanolytic enzymes produced by A. phoenicis showed increased thermostability, with changes in the profiles of pH optima. The chromatographic profiles were distinct when samples obtained from cultures grown at different temperatures were eluted from DEAE-cellulose and Biogel P-60 columns.