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Screening of filamentous fungi for production of enzymes of biotechnological interest

Brazilian Journal of Microbiology (2006) 37:474-480

Luis Henrique S. Guimarães; Simone C. Peixoto-Nogueira; Michele Michelin; Ana Carolina S. Rizzatti; Valéria C. Sandrim; FabianaF. Zanoelo; Ana Carla M.M. Aquino; Altino B. Junior; Maria de Lourdes T.M. Polizeli

Many enzymes produced by fungi have relevant biotechnological applications in several industrial areas. The purpose of this study was to collect and isolate filamentous fungi from soil and humus, plants and sugar cane bagasse of different regions of the São Paulo state. Forty isolates were examined for their ability to produce xylanase, glucose-oxidase, alkaline phosphatase, acid phosphatase, phytase, pectinase and amylase. Among these, twenty three isolates exhibited enzymatic potential. The xylanases produced by two of these isolates (Aspergillus caespitosus and A. phoenicis) showed good potential for pulp bleaching. Among seventeen isolates, at least three produced high levels of glucose-oxidase, being Rhizopus stolonifer and A. versicolor the best producer strains. A. caespitosus, Mucor rouxii, and nine others still not identified were the best