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Production and properties of xylanases from Aspergillus terricola Marchal and Aspergillus ochraceus and their use in cellulose pulp bleaching

M. Michelin • S. C. Peixoto-Nogueira • J. H. A. Betini • T. M. da Silva • J. A. Jorge • H. F. Terenzi • M. L. T. M. Polizeli

Bioprocess Biosyst Eng (2010) 33:813–821

Aspergillus terricola and Aspergillus ochraceus, isolated from Brazilian soil, were cultivated in Vogel and Adams media supplemented with 20 different carbon sources, at 30ºC, under static conditions, for 120 and 144 h, respectively. High levels of cellulase-free xylanase were produced in birchwood or oat spelt xylan-media. Wheat bran was the most favorable agricultural residue for xylanase production. Maximum activity  was obtained at 60ºC and pH 6.5 for A. terricola, and 65ºC and pH 5.0 for A. ochraceus. A. terricola