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Evidence of thermostable amylolytic activity from Rhizopus microsporus var. rhizopodiformis using wheat bran and corncob as alternative carbon source

Bioprocess Biosyst Eng (2008) 31:329–334

S. C. Peixoto-Nogueira, V. C. Sandrim, L. H. S. Guimarães, J. A. Jorge, H. F. Terenzi, M. L. T. M. Polizeli



Rhizopus microsporus var. rhizopodiformis produced high levels of a-amylase and glucoamylase under solid state fermentation, with several agricultural residues, such as wheat bran, cassava flour, sugar cane bagasse, rice straw, corncob and crushed corncob as carbon sources. These materials were humidified with distilled water, tap water, or saline solutions—Segato Rizzatti (SR), Khanna or Vogel. The best substrate for amylase production was wheat bran with SR saline solution (1:2 v/v). Amylolytic activity was still improved (14.3%) with a mixture of wheat bran, corncob, starch and SR saline solution (1:1:0.3:4.6 w/w/w/v). The optimized