JBC Papers in Press. Published on October 17, 2011
Lucas F. Ribeiro, Gilvan P. Furtado, Marcos R. Lourenzoni, Antonio J. Costa-Filho, Camila R. Santos, Simone C. Peixoto Nogueira, Jorge A. Betini, Maria de Lourdes T. M. Polizeli, Mario T. Murakami, Richard J. Ward.
Background - Rational design methods can be used to create chimeric enzymes with novel catalytic combinations. Results. Bifunctional enzymes combining xylanase and laccase activities showed enhanced catalytic activity and stability.
Conclusion - Formation of an interdomain interface alters enzyme conformation which enhances catalytic performance of the chimera.
Significance - Deeper understanding of structural principles of protein fusion can improve the design of novel catalysts.
