Anti-inflammatory effects of atorvastatin: modulation by the T-786C polymorphism in the endothelial nitric oxide synthase gene

Atherosclerosis. 2007; 193(2):438-444

Souza-Costa DC, Sandrim VC, Lopes LF, Gerlach RF, Rego EM, Tanus-Santos JE.

Statins produce cholesterol-independent, anti-inflammatory effects, which result at least in part from increased endothelial nitric oxide production. These effects may be modulated by polymorphisms in the endothelial nitric oxide synthase (eNOS) gene. Here, we examined whether the T-786C polymorphism of eNOS gene affects the concentrations of markers of atherosclerosis and inflammation (sCD40L, sVCAM-1, sICAM-1,

Endothelial nitric oxide synthase genotype and haplotype are not associated with diabetic retinopathy in diabetes type 2 patients.

Nitric Oxide 2006; 15(4):417-22

de Syllos RW, Sandrim VC, Lisboa HR, Tres GS, Tanus-Santos JE.

Polymorphisms in the endothelial nitric oxide synthase (eNOS) gene have been associated with the development of diabetic retinopathy (DR) in patients with type 1 diabetes mellitus (T1DM), but not with T2DM. However, no previous study has analyzed combinations of genetic markers (haplotypes), which can be more informative. We studied three eNOS genetic polymorphisms: a single nucleotide polymorphism

Ethnicity affects the distribution of delta-aminolevulinic acid dehydratase (ALAD) genetic variants

Clin Chim Acta. 2006;367(1-2):192-5

Montenegro MF, Barbosa F Jr, Sandrim VC, Gerlach RF, Tanus-Santos JE.

BACKGROUND: delta-Aminolevulinic acid dehydratase (ALAD) catalyzes the second step of heme synthesis. The ALAD gene shows a polymorphism (G-to-C transversion at position 177) leading to 2 alleles (ALAD1 and ALAD2) and 3 phenotypes (ALAD 1-1, ALAD 1-2 and ALAD 2-2). This polymorphism has been shown to affect lead toxicity and the risk of meningioma. In addition, there is little evidence showing interethnic differences in the distribution this polymorphism, especially in heterogeneous populations such as the present-day Brazilian population. We examined the distribution of genetic variants of the G177C ALAD polymorphism in black and white Brazilians. METHODS: We studied 115 subjects self-reported as black and

Endothelial nitric oxide synthase haplotypes affect the susceptibility to hypertension in patients with type 2 diabetes mellitus.

Atherosclerosis. 2006 ; 189(1):241-6

Sandrim VC, de Syllos RW, Lisboa HR, Tres GS, Tanus-Santos JE.

Department of Pharmacology, Faculty of Medicine of Ribeirao Preto, University of
Sao Paulo, Av. Bandeirantes, 3900, 14049-900 Ribeirao Preto, SP, Brazil.

Type 2 diabetes mellitus (T2DM) and hypertension (HT) commonly coexist. While endothelial nitric oxide synthase (eNOS) haplotypes have been associated with HT, it is unknown whether eNOS genotypes/haplotypes are associated with altered susceptibility to HT in patients with T2DM. We studied the distribution of three eNOS genetic polymorphisms: a single nucleotide polymorphism in the promoter region (T(-786)C), in exon 7 (Glu298Asp), and a variable number of tandem repeats in intron 4(b/a). Genotypes were determined for 102 healthy controls, 119 patients with HT, 66 patients with T2DM, and 113 patients

A polymorphism in the delta-aminolevulinic acid dehydratase gene modifies plasma/whole blood lead ratio

Arch Toxicol. 2006; 80(7):394-8

Montenegro MF, Barbosa F Jr, Sandrim VC, Gerlach RF, Tanus-Santos JE.

Delta aminolevulinic acid dehydratase (ALAD) plays an important role in lead poisoning. This study was carried out to examine the effects of ALAD gene polymorphism (G177C) on %Pb-P(plasma lead)/Pb-B(whole blood) ratio in 142 subjects environmentally exposed to lead. Genotypes for the ALAD G177C polymorphism were determined by PCR and restriction fragment length digestion. Pb-P and Pb-B were determined by inductively coupled plasma mass spectrometry and by graphite furnace atomic absorption spectrometry, respectively. The allele frequencies for ALAD1 and ALAD2 alleles were 0.897 and 0.103, respectively. We combined both ALAD 1-2 and ALAD 2-2 genotypes together (ALAD 1-2/2-2 group) and

Cada vez mais perto do etanol celulósico

Empresa traz para o Brasil enzimas capazes de auxiliar na produção de álcool a partir do bagaço de cana

 

A produção do etanol celulósico - o etanol extraído a partir da biomassa, como o bagaço de cana, por exemplo - pode finalmente ganhar escala industrial. Esse processo, que já é estudado há pelo menos uma década no mundo, pode aumentar em pelo menos um terço a produção de etanol no País, atualmente em 24 bilhões de litros, e tornar mais próxima a consolidação do mercado externo de biocombustíveis.

Reportagem do ESTADÃO - leia mais em: http://www.estadao.com.br/noticias/suplementos,cada-vez-mais-perto-do-etanol-celulosico,531765,0.htm

eNOS genotype-dependent correlation between whole blood lead and plasma nitric oxide products concentrations.

Nitric Oxide. 2006; 14(1):58-64

Barbosa F Jr, Sandrim VC, Uzuelli JA, Gerlach RF, Tanus-Santos JE.

Experimental data indicate that lead exposure decreases nitric oxide (NO) availability. However, no previous study has examined whether lead exposure affects plasma nitrite/nitrate (NO(x)) concentrations in humans. In addition, the T(-786)C polymorphism affects endothelial NO synthase (eNOS) expression and endogenous NO release. Here, we investigated whether there is an association between the circulating concentrations of NO(x) and the concentrations of lead in whole blood (B-Pb) and in plasma (P-Pb) from lead-exposed